The Production of Human β-Glucocerebrosidase in Nicotiana benthamiana Root Culture

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The production of human glucocerebrosidase in glyco‐engineered Nicotiana benthamiana plants

For the production of therapeutic proteins in plants, the presence of β1,2-xylose and core α1,3-fucose on plants' N-glycan structures has been debated for their antigenic activity. In this study, RNA interference (RNAi) technology was used to down-regulate the endogenous N-acetylglucosaminyltransferase I (GNTI) expression in Nicotiana benthamiana. One glyco-engineered line (NbGNTI-RNAi) showed ...

متن کامل

Accumulation of Taxadiene by Root Culture of Nicotiana Benthamiana Domin Transformed with Taxadiene Synthase

Nicotiana benthamiana was transformed with a taxadiene synthase (TS) gene for the de novo synthesis of taxadiene. The successfully transformed lines of N. benthamiana producing taxadiene was further cultivated for root culture to produce taxadiene in in vitro system. The roots of lines TSS-5, TSS-7, TSS-8 and TSS-10 accumulated 14.6 22.5 μg/g (dw) taxadiene. Methyl jasmonate treatment to the TS...

متن کامل

Production of complex multiantennary N-glycans in Nicotiana benthamiana plants.

In recent years, plants have been developed as an alternative expression system to mammalian hosts for the production of therapeutic proteins. Many modifications to the plant glycosylation machinery have been made to render it more human because of the importance of glycosylation for functionality, serum half-life, and the safety profile of the expressed proteins. These modifications include re...

متن کامل

Production of human vitronectin in Nicotiana benthamiana using the INPACT hyperexpression platform

Human vitronectin (hVN) is a glycoprotein that functions as a cell adhesion molecule and a regulator of coagulation in blood plasma and the extracellular matrix. In vitro, hVN is added to serum-free media in order to promote the adhesion of animal cells to tissue culture surfaces and the proliferation of undifferentiated stem cells. Here, we report the production of hVN in Nicotiana benthamiana...

متن کامل

Engineering of human-type O-glycosylation in Nicotiana benthamiana plants

Therapeutic properties of recombinant proteins are very often affected by the composition and heterogeneity of their glycans. Conventional expression systems for recombinant pharmaceutical proteins typically do not address this problem and produce a mixture of glycoforms that are neither identical to human glycans nor optimized for enhanced efficacy. In terms of glycosylation, plants offer cert...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: International Journal of Molecular Sciences

سال: 2018

ISSN: 1422-0067

DOI: 10.3390/ijms19071972